Identification and molecular characterization of oat peptides implicated on coeliac immune
response
Engelsk titel: Identification and molecular characterization of oat peptides implicated on coeliac immune
response
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Författare:
Comino, Isabel
;
Bernardo, David
;
Bancel, Emmanuelle
;
Moreno, Maria de Lourdes
;
Sanchez, Borja
;
Barro, Francisco
;
Suligoj, Tanja
;
Ciclitira, Paul J
;
Cebolla, Ángel
;
Knight, Stella C
;
Branlard, Gerard
;
Sousa, Carolina
Email: csoumar@us.es
Språk: Eng
Antal referenser: 59
Dokumenttyp:
Artikel
UI-nummer: 16033424
Sammanfattning
Background: Oats provide important nutritional and pharmacological properties, although their
safety in coeliac patients remains controversial. Previous studies have confirmed that the reactivity
of the anti-33-mer monoclonal antibody with different oat varieties is proportional to the immune
responses in terms of T-cell proliferation. Although the impact of these varieties on the adaptive
response has been studied, the role of the dendritic cells (DC) is still poorly understood. The aim of
this study is to characterize different oat fractions and to study their effect on DC from coeliac
patients.
Methods and results: Protein fractions were isolated from oat grains and analyzed by SDS-PAGE.
Several proteins were characterized in the prolamin fraction using immunological and proteomic
tools, and by Nano-LC-MS/MS. These proteins, analogous to a- and ?-gliadin-like, showed reactive
sequences to anti-33-mer antibody suggesting their immunogenic potential. That was further
confirmed as some of the newly identified oat peptides had a differential stimulatory capacity on
circulating DC from coeliac patients compared with healthy controls.
Conclusions: This is the first time, to our knowledge, where newly identified oat peptides have been
shown to elicit a differential stimulatory capacity on circulating DC obtained from coeliac patients,
potentially identifying immunogenic properties of these oat peptides.