Resveratrol attenuated hydrogen peroxide-induced myocardial apoptosis by autophagic flux
Sammanfattning
Background: Resveratrol is a Sirt-1-specific activator, which also exerts cardioprotective effects
that regulate redox signalling during oxidative stress and autophagy during cardiovascular disease
(CVD).
Objective: This study investigated the protective effects of resveratrol against hydrogen peroxide-
induced damage in cardiomyocytes.
Design: In this article, hydrogen peroxide-induced autophagy and apoptosis in H9c2 cardiomyoblasts
were studied at an increasing concentration from 0 to 100 µM.
Results: Resveratrol pretreatment with concentrations of 10, 20, and 50 µM inhibits autophagic
apoptosis by increasing p-Akt and Bcl-2 protein levels in H9c2 cells. Interestingly, resveratrol
treatment activates the Beclin-1, LC3, p62, and the lysosome-associated protein LAMP2a within 24 h
of administration.
Conclusions: These results suggest that resveratrol-regulated autophagy may play a role in
degrading damaged organelles in H9c2 cells rather than causing apoptosis, and this may be a
possible mechanism by which resveratrol protects the heart during CVD.