Protective effects of Carissa opaca fruits against CCl4-induced oxidative kidney lipid
peroxidation and trauma in rat
Sammanfattning
Background: Carbon tetrachloride (CCl4) is a potent nephrotoxin, as it causes acute as well as
chronic toxicity in kidneys. Therefore, this study was carried out to assess the pharmacological
potential of different fractions of Carissa opaca fruits on CCl4-induced oxidative trauma in the kidney.
Methods: The parameters studied in this respect were the kidney function tests viz, serum profile,
urine profile, genotoxicity, characteristic morphological findings, and antioxidant enzymatic level of
kidneys.
Result: The protective effects of various fractions of C. opaca fruits against CCl4 administration were
reviewed by rat renal function alterations. Chronic toxicity caused by 8-week treatment of CCl4 to the
rats significantly decreased the pH level, activities of antioxidant enzymes, and glutathione contents,
whereas a significant increase was found in the case of specific gravity, red blood cells, white blood
cells, level of urea, and lipid peroxidation in comparison to control group. Administration of various
fractions of C. opaca fruit with CCl4 showed protective ability against CCl4 intoxication by restoring
the urine profile, activities of antioxidant enzymes, and lipid peroxidation in rat. CCl4 induction in rats
also caused DNA fragmentation and glomerular atrophy by means of dilation, disappearance of
Bowmen’s space, congestion in the capillary loops, dilation in renal tubules, and foamy look of
epithelial cells of tubular region, which were restored by co-admiration of various fractions of C.
opaca.
Conclusion: Results revealed that the methanolic fractions of C. opaca are the most potent and
helpful in kidney trauma.