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Background: The test cells used for indirect antiglobulin technique are diluted with Phosphate Buffered Saline (PBS) in a test tube. Test cells in PBS keep for 24 hours. The Bloodbank in Oslo wanted to find a solution for preserving red cells that prolonged the cells’ shelf life. The purpose of this study was to investigate whether the test cells in an Ec-Stabilizing solution had the same quality as the test cells in PBS, and whether the test cells would be stable for two weeks. Material and methods: 37 samples with 11 antibodies to antigen in the five most common bloodgroup systems were investigated. The cells in the Ec-Stabilizing solution were kept at +22°C or at +4°C between the analyses. The samples were analysed on day 1 (n = 35), day 7 (n = 20) and day 14 (n = 36). All samples were analysed simultaneously with the cells in PBS (n = 91). The strength of agglutination was converted into scores (0 -12 points). Results: Agglutination with the cells in PBS showed lower scores than with the cells in Ec-Stabilizing solution, regardless of whether we looked at all the tests or at each analysis day, and regardless of whether the cells in Ec-Stabilizing solution were kept at +22°C or +4°C. There were no false positive results. Conclusion: Based on our results we found that Ec-Stabilizing solution can be used for IAT in test tubes. The cells have a shelf life of two weeks, both when stored at +22°C and at +4°C.

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