Ameliorating effect of olive oil on fertility of male rats fed on genetically modified soya bean
Sammanfattning
Background: Genetically modified soya bean (GMSB) is a commercialized food. It has been
shown to have
adverse effects on fertility in animal trials. Extra virgin olive oil (EVOO) has many beneficial effects
including
anti-oxidant properties. The aim of this study is to elucidate if addition of EVOO ameliorates the
adverse
effects on reproductive organs of rats fed on GMSB containing diet.
Methods: Forty adult male albino rats (150180 g) of Sprague Dawley strain were separated into four
groups
of 10 rats each: Group 1 control group fed on basal ration, Group 2 fed on basal ration mixed with
EVOO
(30%), Group 3 fed on basal ration mixed with GMSB (15%), and Group 4 fed on basal ration
mixed with
GMSB (15%) and EVOO (30%). This feeding regimen was administered for 65 days. Blood samples
were
collected to analyze serum zinc, vitamin E, and testosterone levels. Histopathological and weight
changes in
sex organs were evaluated.
Results: GMSB diet reduced weight of testis (0.6690.06 vs. 1.790.06, pB0.001), epididymis
(0.48990.03 vs.
0.790.03, pB0.001), prostate (0.0490.009 vs. 0.6890.04, pB0.001), and seminal vesicles (0.05790.01
vs.
0.890.04, pB0.001). GMSB diet adversely affected sperm count (40697.1 vs. 61097.8, pB0.001),
motility
(pB0.001), and abnormality (pB0.001). GMSB diet also reduced serum zinc (pB0.05), vitamin E
(pB0.05),
and testosterone (pB0.05) concentrations. EVOO diet had no detrimental effect. Addition of EVOO to
GMSB diet increased the serum zinc (pB0.05), vitamin E (pB0.05), and testosterone (pB0.05) levels
and also restored the weights of testis (1.3590.16 vs. 0.6690.06, pB0.01), epididymis (0.61490.13 vs.
0.48990.03, pB0.001), prostate (0.29190.09 vs. 0.0490.009, pB0.001), seminal vesicle (0.51690.18
vs.
0.05790.01, pB0.001) along with sperm count (51693.1 vs. 40697.1, pB0.01), motility (pB0.01), and
abnormality (pB0.05).
Conclusion: EVOO ameliorates the adverse effects of GMSB on reproductive organs in adult male
albino
rats. This protective action of EVOO justifies its use against the oxidative damage induced by GMSB
in
reproductive organs