Anti-inflammatory effects of potato extract on a rat model of cigarette smoke - induced chronic
obstructive pulmonary disease
Engelsk titel: Anti-inflammatory effects of potato extract on a rat model of cigarette smoke - induced chronic
obstructive pulmonary disease
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Författare:
Xu, Gui Hua
;
Shen, Jie
;
Sun, Peng
;
Yang, Min Li
;
Zhao, Peng Wei
;
Niu, Yan
;
Lu, Jing Kun
;
Wang, Zhi Qiang
;
Gao, Chao
;
Han, Xue
;
Liu, Lei Lei
;
Liu, Chen Chen
;
Cong, Zhang Yue
Email: nmg_ylm@163.com
Språk: Eng
Antal referenser: 38
Dokumenttyp:
Artikel
UI-nummer: 15123765
Sammanfattning
Objective: This study aimed to evaluate the therapeutic effects of potato extract (PE) on cigarette
smoke (CS)
induced chronic obstructive pulmonary disease (COPD).
Methods: PE was first prepared by frozen centrifugation, and its amino acid composition was
detected. Toxicity
of PE was analyzed by changes in morphology, behavior, routine blood indexes, and biochemical
criteria
of mice. Then, the COPD rat model was established by CS exposure, and PE, doxofylline, and
prednisolone
acetate were used to treat these rats. After 45 days of treatment, the morphology and behavior of rats
were
recorded. In addition, the histopathology of lung tissue was evaluated by chest x-ray and
hematoxylin and
eosin staining. The expression of interleukine-10 (IL-10), tumor necrosis factor-a (TNF-a), and
granulocyte
colony-stimulating factor (G-CSF) was detected in serum and lung tissue by enzyme-linked
immunosorbent
assay (ELISA) and immunohistochemistry, respectively.
Results: Various amino acids were identified in PE, and no toxicity was exhibited in mice. The CS-
induced
COPD rat model was successfully established, which exhibited significant thickened and disordered
lung
markings on 90% of the rats. After administering doxofylline and prednisolone acetate, inflammation
symptoms
were improved. However, side effects such as emaciation, weakness, and loosening of teeth
appeared. In the
PE group, obviously improved histopathology was observed in lung tissues. Meanwhile, it was
revealed that
PE could increase the expression of IL-10 and reduce the expression of TNF-a and G-CSF in COPD
rats, and
doxofylline and prednisolone acetate also elicited similar results.
Conclusion: Our study suggests PE might be effective in the treatment of CS-induced COPD by
inhibiting
inflammation.