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Introduction and purpose - To investigate the influence of relative humidity and application time on bond strength to dentin of different classes of adhesive systems. Material and methods - A total of 360 extracted human molars were ground to mid-coronal dentin. The dentin specimens were treated with one of six adhesive systems (Syntac Classic, OptiBond FL, Clearfil SE Bond, AdheSE, Xeno Select, or Scotchbond Universal) and resin composite (Filtek Z250) was applied to the treated dentin surface under four experimental conditions (45% relative humidity/application time according to manufacturers’ instructions; 45% relative humidity/reduced application time; 85% relative humidity/application time according to manufacturers’ instructions; 85% relative humidity/reduced application time). After storage (37°C, 100% humidity, 24 h), shear bond strength (SBS) was measured and data analysed with nonparametric ANOVA followed by Kruskal-Wallis tests and Mann-Whitney U-tests with Bonferroni-Holm correction for multiple testing (level of significance: a = 0.05). Results and conclusion - Increased relative humidity and reduced application time had no effect on SBS for Clearfil SE Bond and Scotchbond Universal (P = 1.00). For Syntac Classic, OptiBond FL, AdheSE, and Xeno Select there was no effect on SBS of reduced application time of the adhesive system (P = 0.403). However, increased relative humidity significantly reduced SBS for Syntac Classic, OptiBond FL, and Xeno Select irrespective of application time (P = 0.003), whereas for AdheSE, increased relative humidity significantly reduced SBS at recommended application time only (P = 0.002). Generally, increased relative humidity had a detrimental effect on SBS to dentin, but reduced application time had no effect.

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