Low-molecular-weight fucoidan and high-stability fucoxanthin from brown seaweed exert prebiotics and anti-inflammatory activities in Caco-2 cells
Sammanfattning
Background: The aim of this study is to investigate the anti-inflammatory effects of low-molecular-weight
fucoidan (LMF) and high-stability fucoxanthin (HS-Fucox) in a lipopolysaccharide-induced inflammatory
Caco-2 cell line co-culture with B. lactis.
Methods: We used various methods such as transepithelial resistance (TER) assay, cytokine secretion assay,
and tight junction protein mRNA expression assay to examine LMF and HS-Fucox anti-inflammatory
properties.
Results: LMF and HS-Fucox activated probiotic growth and reduced the inflammation of the intestinal
epithelial cells. Moreover, the combination of LMFHS-Fucox dramatically enhanced the intestinal epithelial
barrier and immune function against the lipopolysaccharide effect by inhibiting IL-1b and TNF-a and
promoting IL-10 and IFN-g.
Conclusion: These findings suggested that LMF and HS-Fucox, alone or in combination, could be the
potential natural compounds to enhance the immune system and have an anti-inflammatory effect on the
intestinal cells