Enhanced polyunsaturated fatty acids production in Mortierella alpina by SSF and the enrichment in chicken breasts
Engelsk titel: Enhanced polyunsaturated fatty acids production in Mortierella alpina by SSF and the enrichment in chicken breasts
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Författare:
Yang, Shegli
;
Zhang, Hui
Email: zhanghui0461@sina.com.cn
Språk: Eng
Antal referenser: 58
Dokumenttyp:
RCT
UI-nummer: 17050116
Sammanfattning
Background: Distiller’s dried grains with solubles (DDGS) and soybean meal were used as the substrates
for the production of polyunsaturated fatty acids (PUFA) in solid-state fermentation (SSF) by Mortierella
alpine. These fermented products were fed to laying hens. PUFA enrichment from chicken breasts was
studied.
Methods: The maximum productivity of PUFA was achieved under optimized process condition, including 1%
w/w yeast extract as additive, an incubation period of 5 days at 128C, 10% v/w inoculum level, 75% moisture
content, and pH 6.0. The hens were then fed with ration containing soybean DDGS, rapeseed oil, soybean oil,
and peanut oil. The control group was fed with basal ration.
Results: Under the optimal condition, M. alpine produced total fatty acids (TFA) of 182.34 mg/g dry substrate.
It has better mycelial growth when soybean meal was added to DDGS (SDDGS). PUFA in fermentation
product increased with higher soybean meal content. The addition of 70% soybean meal to DDGS substrate
yielded 175.16 mg of TFA, including 2.49 mg eicosapentaenoic acid (EPA) and 5.26 mg docosahexaenoic acid
(DHA). The ratios of v-6/v-3 found in chicken breasts fat were all lower than that found in control by 36.98,
31.51, 18.15, and 12.63% for SDDGS, rapeseed oil, soybean oil, and peanut oil, respectively.
Conclusions: This study identified an optimized SSF process to maximize PUFA productivity by M. alpine as
the strain. This PUFA-enriched feed increased the PUFA contents as well as the proportions of v-6 and v-3
in chicken breasts and liver.