Effects of stachyose on absorption and transportation of tea catechins in mice: possible role of Phase II metabolic enzymes and efflux transporters inhibition by stachyose
Engelsk titel: Effects of stachyose on absorption and transportation of tea catechins in mice: possible role of Phase II metabolic enzymes and efflux transporters inhibition by stachyose
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Författare:
Li, Wenfeng
;
Lu, Yalong
;
Huang, Di
;
Han, Xiao
;
Yang, Xingbin
Email: xbyang@snnu.edu.cn
Språk: Eng
Antal referenser: 33
Dokumenttyp:
Artikel
UI-nummer: 17070028
Sammanfattning
Background: Nutritional and absorption-promoting properties of stachyose combined with tea catechins (TC)
have been revealed. However, the mechanism involved in non-digestible oligosaccharides-mediated enhancement
of flavonoid absorption has largely remained elusive.
Methods: This study was designed to investigate the molecular mechanism of stachyose in enhancing
absorption and transportation of TC in mice. Mice were orally pre-treated with stachyose (50, 250, and 500
mg/kgbw) for 08 weeks, and 1 h before sacrifice, mice were treated with TC (250 mg/kgbw).
Results: Gas chromatography-mass spectrometry analysis showed that serum concentrations of epicatechin,
epigallocatechin, epicatechin gallate, and epigallocatechin gallate were dose- and time-dependently elevated
with stachyose pre-treatment in mice. Furthermore, pre-treatment with stachyose in mice reduced intestinal
sulfotransferase and uridine diphosphate-glucuronosyltransferase levels by 3.343.2% and 23.930.4%,
relative to control mice, respectively. Moreover, intestinal P-glycoprotein and multidrug resistance-associated
protein-1 contents were decreased in mice by pre-administration of stachyose in dose- and time-dependent
manner.
Conclusions: This is the first time to demonstrate that suppression of Phase II metabolic enzymes and efflux
transporters of TC in the intestine can play a major role in increasing absorption of TC by stachyose feeding.