Cpt1a gene expression in peripheral blood mononuclear cells as an early biomarker of diet-related metabolic alterations
Engelsk titel: Cpt1a gene expression in peripheral blood mononuclear cells as an early biomarker of diet-related metabolic alterations
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Författare:
Díaz-Rúa, Rubén
;
Palou, Andreu
;
Oliver, Paula
Email: andreu.palou@uib.es
Språk: Eng
Antal referenser: 43
Dokumenttyp:
Artikel
UI-nummer: 17070039
Sammanfattning
Background: Research on biomarkers that provide early information about the development of future
metabolic alterations is an emerging discipline. Gene expression analysis in peripheral blood mononuclear
cells (PBMC) is a promising tool to identify subjects at risk of developing diet-related diseases.
Objective: We analysed PBMC expression of key energy homeostasis-related genes in a time-course analysis in
order to find out early markers of metabolic alterations due to sustained intake of high-fat (HF) and highprotein
(HP) diets.
Design: We administered HF and HP diets (4 months) to adult Wistar rats in isocaloric conditions to a
control diet, mainly to avoid overweight associated with the intake of hyperlipidic diets and, thus, to be able to
characterise markers of metabolically obese normal-weight (MONW) syndrome. PBMC samples were
collected at different time points of dietary treatment and expression of relevant energy homeostatic genes
analysed by real-time reverse transcription-polymerase chain reaction. Serum parameters related with
metabolic syndrome, as well as fat deposition in liver, were also analysed.
Results: The most outstanding results were those obtained for the expression of the lipolytic gene carnitine
palmitoyltransferase 1a (Cpt1a). Cpt1a expression in PBMC increased after only 1 month of exposure to both
unbalanced diets, and this increased expression was maintained thereafter. Interestingly, in the case of the HF
diet, Cpt1a expression was altered even in the absence of increased body weight but correlated with alterations
such as higher insulin resistance, alteration of serum lipid profile and, particularly, increased fat deposition in
liver, a feature characteristic of metabolic syndrome, which was even observed in animals fed with HP diet.
Conclusions: We propose Cpt1a gene expression analysis in PBMC as an early biomarker of metabolic
alterations associated with MONW phenotype due to the intake of isocaloric HF diets, as well as a marker of
increased risk of metabolic diseases associated with the intake of HF or HP diets