Lipolysis stimulating peptides of potato protein hydrolysate effectively suppresses high-fat-diet-
induced hepatocyte apoptosis and fibrosis in aging rats
Sammanfattning
Background: Non-alcoholic fatty liver disease (NAFLD) is one of the most common outcomes of
obesity and is characterized by the accumulation of triglycerides, increased tissue apoptosis, and
fibrosis. NAFLD is more common among elderly than in younger age groups, and it causes serious
hepatic complications.
Objective: In this study, alcalase treatment derived potato protein hydrolysate (APPH) with lipolysis-
stimulating property has been evaluated for its efficiency to provide hepato-protection in a high-fat-
diet (HFD)-fed aging rats.
Design: Twenty-four-month-old SD rats were randomly divided into six groups (n=8): aged rats fed
with standard chow, HFD-induced aged obese rats, HFD with low-dose (15 mg/kg/day) APPH
treatment, HFD with moderate (45 mg/kg/day) APPH treatment, HFD with high (75 mg/kg/day) APPH
treatment, and HFD with probucol.
Results: APPH was found to reduce the NAFLD-related effects in rat livers induced by HFD and all of
the HFD-fed rats exhibited heavier body weight than those with control chow diet. However, the HFD-
induced hepatic fat accumulation was effectively attenuated in rats administered with low (15
mg/kg/day), moderate (45 mg/kg/day), and high (75 mg/kg/day) doses of APPH. APPH oral
administration also suppressed the hepatic apoptosis- and fibrosis-related proteins induced by HFD.
Conclusions: Our results thus indicate that APPH potentially attenuates hepatic lipid accumulation
and anti-apoptosis and fibrosis effects in HFD-induced rats. APPH may have therapeutic potential in
the amelioration of NAFLD liver damage.