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Diagnostikk av medfödte kryptiske kromosomavvik
Engelsk titel: Diagnosis of cryptic chromosome aberrations Läs online Författare: Ness GO ; Houge G Språk: Nor Antal referenser: 30 Dokumenttyp: Översikt UI-nummer: 03091396

Tidskrift

Tidsskrift for Den Norske Laegeforening 2003;123(17)2418-21 ISSN 0029-2001 E-ISSN 0807-7096 KIBs bestånd av denna tidskrift Denna tidskrift är expertgranskad (Peer-Reviewed)

Sammanfattning

INTERPRETATION : Though the sensitivity of comparative genomic hybridisation is still insufficient for finding a deletion in most of the well-known microdeletion syndromes, the diagnostic yield of genomic imbalances is better than for all other laboratory investigations except routine G-banding. RESULTS : Using comparative genomic hybridisation with high-resolution analysis, we were able to detect a chromosome aberration in 10 % of patients found to have normal karyotypes by standard chromosome analysis. MATERIALS AND METHODS : We present the current molecular cytogenetic methods for detection of cryptic chromosome aberrations, with special emphasis on comparative genomic hybridisation (CGH), a DNA-based screening method. BACKGROUND : Cryptic chromosome aberrations, i.e. those that are not observed by routine chromosome analysis (G-banding), can cause a plethora of developmental abnormalities, usually at least a combination of various dysmorphic signs and mental retardation.